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The critically acclaimed laboratory standard for more than forty years, "Methods in Enzymology" is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today - truly an essential publication for researchers in all fields of life sciences. This volume and its companion Volumes 279, 280, and 281 provide: a collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes; details on physical, chemical, and biological properties of vitamins and coenzymes; chemical and biological syntheses of vitamins, coenzymes, and their analogs; and aspects of transport and metabolism of vitamins and coenzymes.