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Cancer is the leading cause of death in economically developed countries and the second leading cause of death in developing countries. Natural products are having a long history of use in the service of mankind for the prophylaxis and treatment of several diseases and cancer is not an exception.Triticum aestivum (Wheatgrass) has been traditionally used to treat various disorders and also used as a health tonic. 'WHOLE PLANTS FOR WHOLE PEOPLE' might be a suitable slogan to advertise the principles and practice of medical herbalism. In present study, aqueous extract, methanolic extract and isolated constituents were tested on various cancer cell lines like Hela, HEP- 3B, HCT- 15, HEp- 2 and the results were compared with the Vero cell line. Colchicine is taken as the reference standard.