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In this work Zinc Oxide Nanoparticles have been synthesized by Hydrothermal method using Zinc Acetate Dehydrate, Sodium Hydroxide and Methanol. This synthesis was done in Teflon lined autoclaves at high temperature and high pressure and we get the white solid powder from the stock solution. Quantitative result of the samples was studied by Energy Dispersive X-Ray Spectroscopy. The crystal structure and the grain size of the particles was determined from the X-ray Diffraction.