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This book describes the fabrication of a frequency-based electronic tongue using a modified glassy carbon electrode (GCE), opening a new field of using organic precursors for nanostructure growth. It also presents a new approach to optimizing nanostructures via statistical analysis. The chemical vapor deposition (CVD) method was utilized to grow vertically aligned carbon nanotubes (CNTs) with various aspect ratios. To increase the graphitic ratio of synthesized CNTs, sequential experimental strategies based on response surface methodology were employed to investigate the crystallinity of CNTs. In the next step, glucose oxidase (GOx) was immobilized on the optimized multiwall carbon nanotubes/gelatin (MWCNTs/Gl) composite using the entrapment technique to achieve enzyme-catalyzed oxidation of glucose at anodic potentials, which was drop-casted onto the GCE. The modified GCE indicates that a GOx/MWCNTs/Gl/GC electrode can be utilized as a glucose biosensor with a high direct electron-transfer rate between GOx and MWCNTs/Gl. It was possible to use the fabricated biosensor as an electronic tongue thanks to a frequency-based circuit attached to the electrochemical cell. The results indicated that, the modified GCE with the GOx/MWCNTs/Gl showed application potential for glucose in a voltametric- based electronic tongue.