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Molecular investigations on archival tissues
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Nowadays there are many available molecular methods §for studying diseases. Archival tissue provides an §extremely valuable material for proper understanding §of pathology of various diseases. A major problem in §molecular analysis of archival tissues is the tissue §degradation that might occur during long storage. §This monograph presents various methods for §efficient extraction of DNA and protocols for §sequencing (detection of gene mutations) from §archival tissues. It also provides protocols for §immunodetection of various proteins on such archival §tissues collected at Gades Institute, Haukeland §University Hospital, Norway in the period 1932 till §1996. In addition this book illustrates the value of §studies on archival tissues for detecting changes in §the pathogenesis of diseases with time. In this §monograph bladder cancer is used as an example for §such studies. This book could be useful for §professionals, considering the use of archival §tissue to study mechanisms of various diseases.
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