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Our current investigation verifies for the first time, the protective effects of Polygonum bistorta and Tannic Acid against model hepatotoxicant in rats. The hepatoprotective action is likely related to its potent antioxidative and anti-inflammatory activity. Neutralizing reactive oxygen species by non enzymatic mechanism and enhancing the activity of original natural hepatic antioxidants enzymes may be the main mechanisms against injury. These data provide a scientific explanation for the folkloric uses of Polygonum bistorta and tannic acid in the treatments of hepatic disorders. The findings provide a rationale for further studies on pharmacological evaluation. Furthermore additional studies on mechanism of action at molecular level are required. This data provides a scientific explanation for the folkloric uses of Polygonum bistorta and tannic acid in the treatments of hepatic disorders.