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The aim of this book is to provide consolidated information on the aspect of enzyme engineering. It presents essential information not only for undergraduate and graduate students, but also for the people in academia and industry in their quest for the commercial exploitation of potential enzymes. A brief history of enzyme technology is incorporated to present the milestones of enzyme advancement. The fundamentals of enzyme chemistry, biosynthesis of enzyme and its production are described to provide an insight on where and how the enzyme is produced. Enzyme's stability and reaction kinetics during enzymatic reactions are presented to elaborate on how the enzyme functions during catalysis and what the factors are that affect its activity. Methods to improve enzyme performance are also presented book such as cofactors regeneration and enzyme immobilization. The book will emphasize and elaborate the performance and characteristics of the enzyme at the molecular level. Lastly, the book presents recent advances in enzyme engineering and the industrial application of enzymes to answer the present needs of the society.