Effects of Src-inhibitors on osteoclasts in vitro

The human skeleton is continuously regenerated in §order to repair damages of the bone and to maintain §homeostasis. Disturbances in the process of §remodelling can give rise to common bone disorders §such as osteoporosis. In Osteoporosis the resorption §of bone exceeds the formation of new bone resulting §in loss of bone mass and fractures. Many people §suffer from osteoporosis and other bone diseases and §therefore it is important to search for effective §pharmaceutical compounds. The Src inhibitor §substances SI35, SI34, SI83, SI24, and S2 were §studied for their effects on osteoclasts and bone §remodelling in vitro. The aim was to investigate §their functions on osteoclastic bone resorption §activity and osteoclast differentiation from §precursors. Also the effects on viability and the §number of osteoclasts were examined. Src inhibitor §substance SI83 had the strongest inhibitory §activity in the resorption assays with coculture-§derived osteoclasts as well as in the resorption §assays with rabbit osteoclasts. All of the tested §substances had an effect on the differentiation of §osteoclasts derived from mouse coculture. The human skeleton is continuously regenerated in §order to repair damages of the bone and to maintain §homeostasis. Disturbances in the process of §remodelling can give rise to common bone disorders §such as osteoporosis. In Osteoporosis the resorption §of bone exceeds the formation of new bone resulting §in loss of bone mass and fractures. Many people §suffer from osteoporosis and other bone diseases and §therefore it is important to search for effective §pharmaceutical compounds. The Src inhibitor §substances SI35, SI34, SI83, SI24, and S2 were §studied for their effects on osteoclasts and bone §remodelling in vitro. The aim was to investigate §their functions on osteoclastic bone resorption §activity and osteoclast differentiation from §precursors. Also the effects on viability and the §number of osteoclasts were examined. Src inhibitor §substance SI83 had the strongest inhibitory §activity in the resorption assays with coculture-§derived osteoclasts as well as in the resorption §assays with rabbit osteoclasts. All of the tested §substances had an effect on the differentiation of §osteoclasts derived from mouse coculture.