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The book gives students and young researchers a resource on zinc enzymes, some of which were intensively studied for more than six decades and translated into model success stories of the pharmaceutical industry. Up-to-date overviews on the major drug targets of the field are presented, showing the structural basis for catalysis, binding of inhibitors and activators, and their clinical applications. Emergent roles of some recently discovered isozymes in cancer, obesity, epilepsy, pain management, and malaria are also introduced, together with the most recent achievements in drug design from both academia and industry.