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This volume of "Methods in Enzymology" covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications. It is extensively referenced, with useful figures and tables throughout the volume. It is written by expert authors who are leaders in the field. It is extensively referenced and has useful figures and tables. It provides hints and tricks to facilitate reproduction of methods.