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This book constitutes the proceedings of the 5th InternationalConference on Algorithms for Computational Biology, AlCoB 2018, held in Hong Kong, China, in June 2018. The 11 full papers presented together with 1 invited paper were carefully reviewed and selected from 20 submissions. They are organized in the following topical sections: Phylogenetics, Sequence Rearrangement and Analysis, Systems Biology and Other Biological Processes.